Refining Your Cultures From Spores For Making Grain Spawn, Liquid Culture and Expanding.
- Step # 1: Ounce your plates start showing signs of growth and the spores have germinated, not all your plates will turn out nicely some may be bacterial or show signs of mold growth any plates with mold growing on them can be tossed plates that are not severally bacterial can be saved. An ideal spore plate or dish will have no signs of contamination but this is why we refine our spore cultures.
- Step # 2: Take your nicest plates with the best growth on them. One nice spore plate or container can make several new ones. Select the nicest looking mycelia on the plate (rhizomorphic growth) (Mycelia that looks similar to plant routes is usually the nicest strongest mycelia but this is not always achieved straight from spores you may end up with fuzzy mycelia called tamentose mycelia. https://www.google.com/search?client=firefox-b-d&q=tamentose+mycelia+growth+vs+rhizomorphic
- Step # 3: Ounce you have decided which plates you wish to transfer place them in your SAB or in front of your laminar flow hood. Sterilize your scalpel or exacto blade with a torch till red hot. When transferring your cultured mycelia all you need is a pin sized piece of mycelia from your plate I would recommend a .5cm X .5cm square piece of cultured agar. Partially remove your pp5 container lid or petri dish lid and take your specimen from your plate and transfer it to a new petri dish or pp5 container try to get the specimen within the center of the culturing vessel this is not always possible though.
- Step # 4: Place your newly cultured dish in your clean room temperature area for your cultures and allow some time for growth before repeating the above steps one more time.
- *This is generally done 3 times to ensure clean viable cultured specimens for grain spawn production, later on clones can be taken from the fruits of this mycelia and further cultured for more ideal growth, max yield and ideal size.